Search results for: immunoproteomics

Immunoproteomics

Author : Kelly M. Fulton
File Size : 84.45 MB
Format : PDF
Download : 708
Read : 1252
Download »
This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field.

Immunoproteomics

Author : Kelly M. Fulton
File Size : 28.14 MB
Format : PDF, ePub, Docs
Download : 608
Read : 1092
Download »
This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field. .

Author :
File Size : 24.38 MB
Format : PDF, Kindle
Download : 721
Read : 376
Download »

Immunoproteomics

Author : Peng Li
File Size : 90.95 MB
Format : PDF
Download : 555
Read : 174
Download »

Exploring Humoral Responses During Staphylococcus Aureus Infection by Immunoproteomics

Author : Nandakumar Sundaramoorthy
File Size : 38.1 MB
Format : PDF, ePub, Docs
Download : 493
Read : 962
Download »

Immunoproteomics

Author :
File Size : 56.58 MB
Format : PDF, Mobi
Download : 836
Read : 501
Download »

Immunoproteomics

Author : Peng Li
File Size : 75.58 MB
Format : PDF, ePub, Docs
Download : 681
Read : 1219
Download »

Immunoproteomics

Author :
File Size : 36.78 MB
Format : PDF, Docs
Download : 622
Read : 571
Download »

Application of Immunoproteomics and Bioinformatics to Coccidioidomycosis Vaccinology

Author : Eric James Tarcha
File Size : 36.93 MB
Format : PDF, ePub
Download : 300
Read : 721
Download »
Coccidioides is a primary fungal pathogen endemic to the alkaline desert soil of the Southwestern United States and the etiological agent of coccidioidomycosis (Valley fever), a respiratory disease in humans. Coccidioides represents the only fungal pathogen on the Center for Disease Control's select agent list of possible weapons of bioterrorism, and can cause significant morbidity in infected individuals. Thus, the need for a human vaccine against coccidioidomycosis has come to the forefront. Work has centered on identification and characterization of recombinant T cell-reactive antigens, because clinical and experimental data suggest that activation of a durable MHC II restricted T cell Th1 immune response is of principal importance in establishing durable protective immunity. Immunoprotection experiments in mice using single recombinant vaccine proteins of Coccidioides have resulted in less than optimal survival and clearance of the fungus from the infected host compared to whole cell or multicomponent subcellular vaccines, indicating a lack of protective epitopes in the single protein vaccines. Therefore, it is likely that a protective recombinant protein subunit human vaccine against Coccidioides will contain genetically unrestricted ("promiscuous") protective T cell epitopes, and will most likely be multivalent in nature. In this study we describe an immunoproteomic and bioinformatic approach for profiling a diverse immunogenic protein component of the coccidioidal parasitic cell wall. A phospholipase B (Plb), alpha-mannosidase (Amn1), and an aspartyl protease (Pep1) were selected as candidate vaccine proteins on the basis of their immunogenicity, cellular localization, predicted promiscuous T cell epitope content, and T cell reactivity. These antigens were evaluated individually, and in combination, for their protective efficacy in a pulmonary murine model of infection. Each individual protein showed significant protection in infected mice as evaluated by survival after lethal challenge (53%-61% survival). A combinatorial vaccine composed of all three protective antigens enhanced survival in infected mice (86% survival) and significantly improved clearance of the pathogen from lungs of surviving mice by 90 d post-challenge. This strategy has been successful in producing the most comprehensive profile of immunogenic coccidioidal cell wall antigens to date, and lays the groundwork for the development of an epitope-driven, multivalent human vaccine against coccidioidomycosis.

Immunoproteomics of Rous Sarcoma Surface Proteome Vis a vis Peptide Mass Fingerprints and Biomarkers for Detection of Leukosis sarcoma Virus Infection in Chicken

Author : R. M. Ralte
File Size : 39.16 MB
Format : PDF, Kindle
Download : 199
Read : 1330
Download »

Quantitative proteomics and immunoproteomics to explore bost and Candida albicans complex interplay

Author : Catarina Oliveira Vaz
File Size : 65.49 MB
Format : PDF, Mobi
Download : 328
Read : 1118
Download »
Candida albicans forma parte de la microbiota de individuos sanos. Sin embargo, ante un desequilibrio de la microbiota o inmunosupresión, se puede desarrollar una infección, siendo la candidiasis invasora (IC) un problema clínico relevante. Por esto, el estudio de la interacción hospedador-patógeno es crucial para conocer cómo el sistema inmunitario responde a Candida.Los macrófagos son células inmunes implicadas en el reconocimiento, fagocitosis y destrucción del hongo. Además, se ha descrito la producción de anticuerpos frente a proteínas de C. albicans durante estas infecciones. La proteómica proporciona información de la abundancia de proteínas y de las modificaciones postraduccionales durante la interacción patógeno-hospedador. Así mismo, el análisis de las proteínas secretadas por C. albicans también es importante debido al papel que tienen éstas en la interacción con el hospedador. El estudio de estas proteínas mediante inmunoproteómica resulta útil para el descubrimiento de biomarcadores para el diagnóstico de las IC.

Utilising Quantitative Immunoproteomics to Reveal Differential Autoantibody Biomarker Panels in Serous Ovarian Cancer Patients

Author : Karina Martin
File Size : 89.42 MB
Format : PDF, ePub, Mobi
Download : 977
Read : 800
Download »
Epithelial ovarian cancer accounts for 5% of all cancer deaths and greater than 50% of all gynaecological cancer deaths. It presents at a late clinical stage in more than 60% of patients, and is associated with a 5-year survival of only 30% in this group. In contrast, the 5-year survival for patients with organ-confined stage I ovarian cancer exceeds 90%, and most patients are cured of their disease. Thus, the detection of early stage ovarian cancer is the best way to improve survival. No clinically applicable method exists for the early detection of ovarian cancer. Hence, there is an unmet medical need for an accurate screening test. Most scientific efforts towards early detection are focused on the discovery of tumour-associated antigens (TAA). Autologous antibodies against TAAs, however, may serve as more sensitive diagnostic markers. They circulate in the blood before TAAs and are usually more abundant than the TAAs themselves as a result of amplification through the humoral immune response. Accumulating evidence also suggests that a humoral response already exists during malignant transformation when aberrant gene expression is translated into premalignant cellular changes. In this thesis, potential autoantibody biomarkers for ovarian cancer were discovered, verified and validated as an early detection test. A new immunoproteomic strategy was developed to identify novel autoantibodies that were elevated in serous ovarian cancer patients. Lysate extracted from the ovarian tissue of a patient was applied to an immunoaffinity column generated with autologous antibodies and a paired control immunoaffinity column. Relative quantification of captured autoantigens was performed using isotope coded protein label (ICPL) technology coupled with high resolution LC-MS. At a protein ratio cut-off of 1.45-fold, 148 autoantibodies were found to be enriched in ovarian cancer patients compared to the corresponding controls. Upon bioinformatic prioritisation 50 autoantibody candidates were selected for verification. Protein microarray analysis of 98 samples revealed 9 autoantibody candidates to be significantly different in early stage cancer patients compared to healthy and benign controls. Biomarker candidates anti-ANXA1, anti-SAHH and anti-ARP3 showed the greatest potential where each marker achieved greater than 90% specificity at 83.3% sensitivity. As a 4-biomarker panel with the 'gold standard' for ovarian cancer detection, cancer antigen (CA)125, a sensitivity of 76.5% at 100% specificity was attained. These values of sensitivity and specificity for early stage ovarian cancer surpassed the minimum requirements for an implementable screening test and showed great promise as a diagnostic tool. Validation of the top three autoantibody candidates using protein microarray revealed anti-ANXA1 to be the most robust and effective biomarker for stage I cancer detection. As a single biomarker anti-ANXA1 had 81.8% sensitivity and 71.9% specificity for stage I cancer compared to healthy and benign controls. Excitingly, in combination with CA125, a sensitivity of 71.4% at 100% specificity was achieved when differentiating stage I cancer patients from healthy individuals. For this level of effectiveness a positive and negative predictive value of 100% and 99.99% was achieved, respectively. Therefore, a biomarker panel containing anti-ANXA1 and CA125 may enable the development of a detection test that can be used to screen for stage I serous ovarian cancer in the general population. This promising discovery demands further investigation where continuing analysis in prospective samples is essential. The discovery of a screening test is crucial to reduce the morbidity and mortality caused by ovarian cancer. This study investigates the presence of differential autoantibody signatures in serous ovarian cancer patients as potential biomarkers. Additionally, those identified TAAs that are functionally involved in carcinogenesis could also serve as therapeutic targets. Finally, the immunoproteomic approach developed here could be used in studies aiming to discover novel autoantibody biomarkers for other asymptomatic malignancies.

Biochemistry and Molecular Biology Compendium

Author : Roger L. Lundblad
File Size : 90.99 MB
Format : PDF
Download : 831
Read : 265
Download »
This book is an accessible resource offering practical information not found in more database-oriented resources. The first chapter lists acronyms with definitions, and a glossary of terms and subjects used in biochemistry, molecular biology, biotechnology, proteomics, genomics, and systems biology. There follows chapters on chemicals employed in biochemistry and molecular biology, complete with properties and structure drawings. Researchers will find this book to be a valuable tool that will save them time, as well as provide essential links to the roots of their science. Key selling features: Contains an extensive list of commonly used acronyms with definitions Offers a highly readable glossary for systems and techniques Provides comprehensive information for the validation of biotechnology assays and manufacturing processes Includes a list of Log P values, water solubility, and molecular weight for selected chemicals Gives a detailed listing of protease inhibitors and cocktails, as well as a list of buffers

Advanced Concepts in Human Immunology Prospects for Disease Control

Author : Pooja Jain
File Size : 48.54 MB
Format : PDF, ePub, Mobi
Download : 468
Read : 289
Download »
This book highlights information derived primarily from clinical samples, with particular reference to theoretical and scientific aspects of the human immune system. This text will focus on topics that range from host-pathogen interactions in infectious disease to host immune response in cancer, allergic diseases, neuroinflammatory diseases, and autoimmune disorders. The reader will also have a well-rounded understanding of the behavior of the immune system with particular emphasis on the role of immunoproteomics in immunotherapy, neuroprotective immunity for neurodegenerative and neuroinfectious disease, leukemia-associated dendritic cell induction of adaptive immunity dysregulation, and the role of immune checkpoint inhibitors in cancer, infection, as well as neuroinflammation. Taken together, the contents of this book are intended for both clinicians and researchers in academia and industry.

Spectral Techniques In Proteomics

Author : Daniel S. Sem
File Size : 53.36 MB
Format : PDF, ePub, Docs
Download : 955
Read : 460
Download »
Facilitating the innovation, development, and application of new spectroscopic methods in proteomics, Spectral Techniques in Proteomics provides a broad overview of the spectroscopic toolbox that can be used, either with proteome or sub-proteome mixtures or with individual/purified proteins studied in parallel. It gives a modest overview of

Francisella tularensis and tularemi

Author :
File Size : 28.45 MB
Format : PDF, Docs
Download : 492
Read : 253
Download »

Methods in Animal Proteomics

Author : David Eckersall
File Size : 70.42 MB
Format : PDF
Download : 712
Read : 1096
Download »
The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.

Issues in Life Sciences Muscle Membrane and General Microbiology 2011 Edition

Author :
File Size : 42.25 MB
Format : PDF
Download : 976
Read : 846
Download »
Issues in Life Sciences: Muscle, Membrane, and General Microbiology: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Life Sciences—Muscle, Membrane, and General Microbiology. The editors have built Issues in Life Sciences: Muscle, Membrane, and General Microbiology: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Life Sciences—Muscle, Membrane, and General Microbiology in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Life Sciences: Muscle, Membrane, and General Microbiology: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Proteomics for Studying Foodborne Microorganisms and Their Impact on Food Quality and Human Health

Author : Rosa Anna Siciliano
File Size : 40.91 MB
Format : PDF, ePub, Docs
Download : 319
Read : 1256
Download »
Foodborne microorganisms play a pivotal role in mediating the tight relation between food and human health from a dual perspective. In fact, notwithstanding the introduction of strict regulations and new technologies to ensure food quality and safety, foodborne pathogens continue to cause infections and diseases and represent a serious public health concern, while spoilage bacteria can severely affect food quality thus leading to major industry and commercial losses. On the other hand, probiotics positively affect human health, promoting digestion and uptake of dietary nutrients, strengthening intestinal barrier function, modulating immune response and enhancing antagonism towards pathogens. Nowadays, proteomics represents a key discipline to perform high-throughput studies in the field of food microbiology and enables the most accurate identification of complex networks of proteins involved in the cell adaptation to different growth conditions as well as in beneficial or harmful effects on human health. The present eBook offers an overview of the most up to date proteomic methodologies and further assesses the strength of proteomics in exploring different aspects of the foodborne microorganism life-style and defining biomarkers of food quality and safety.

Peptic Ulcers New Insights for the Healthcare Professional 2012 Edition

Author :
File Size : 85.38 MB
Format : PDF
Download : 151
Read : 284
Download »
Peptic Ulcers: New Insights for the Healthcare Professional / 2012 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Peptic Ulcers in a compact format. The editors have built Peptic Ulcers: New Insights for the Healthcare Professional / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Peptic Ulcers in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Peptic Ulcers: New Insights for the Healthcare Professional / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.