Search results for: multifaceted-protocol-in-biotechnology

Multifaceted Protocol in Biotechnology

Author : Azura Amid
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This edited work presents useful methods in experimenting in the area of Bioprocessing and Biotechnology. The four sections cover the area of Bioprocess, Whole Cells & Isolated Biocatalyst, Characterization of Biochemical Products and Cell Isolation & Culture. Its enable researchers to choose a suitable method and plan their experiments in details. The main focus of this book is to provide step by step method to young researchers, especially in the new research areas. Among the latest areas are the isolation of novel strain or enzyme by metagenomic approaches and taming procedure in the laboratories, development of novel, the cheap and non-toxic catalyst for biodiesel production, and production of micro-fibrillated cellulose. An updated method for well-known areas such as immobilization technology, biosensor, and polymerization was also presented. The book also covers in-silico methods such as MATLAB platform to ease researchers. Not to forget, the method in animal and plant culture is also discussed in detail. The book is written by chapter authors with much expertise in their fields. They have published multiple articles in the index listed journals. The topic of this book is particularly relevant to young researchers who are struggling to fine-tune their research and do not want to waste their time in optimizing the experiment set up.

Laccases in Bioremediation and Waste Valorisation

Author : Dietmar Schlosser
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This Microbiology Monographs volume covers the latest advances in laccase applications in bioremediation and waste valorisation. The first three chapters provide a comprehensive introduction to fungal and bacterial laccases (the two most important enzyme groups from an application viewpoint) and their practical use in bioremediation and lignocellulosic waste valorisation. Subsequent chapters discuss possible combinations of laccases and further potentially collaborating enzymes, and offer in-depth insights into laccase immobilisation for wastewater treatment and environmental biosensor applications of laccases. Lastly, the book addresses the quest for enzymes with improved and better-fitting properties, covering laccase engineering by directed and computational evolution, and novel enzymes from extreme environments. As such, it is a fascinating read for microbiologists in both industry and academia.

Advances in Nanotechnology and Its Applications

Author : Ahmad Tariq Jameel
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Multifaceted Roles of Crystallography in Modern Drug Discovery

Author : Giovanna Scapin
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The present work offers a snapshot of the state-of-the-art of crystallographic, analytical, and computational methods used in modern drug design and development. Topics discussed include: drug design against complex systems (membrane proteins, cell surface receptors, epigenetic targets, and ribosomes); modulation of protein-protein interactions; the impact of small molecule structures in drug discovery and the application of concepts such as molecular geometry, conformation, and flexibility to drug design; methodologies for understanding and characterizing protein states and protein-ligand interactions during the drug design process; and monoclonal antibody therapies. These methods are illustrated through their application to problems of medical and biological significance, such as viral and bacterial infections, diabetes, autoimmune disease, and CNS diseases. As approaches to drug discovery have changed over time, so have the methodologies used to solve the varied, new, and difficult problems encountered in drug discovery. In recent years we have seen great progress in the fields of genetics, biology, chemistry, and medicine, but there are still many unmet medical needs, from bacterial infections to cancer to chronic maladies, that require novel, different, or better therapies. This work will be of interest to researchers and policy makers interested in the latest developments in drug design.

Proteomics as a Multifaceted Tool in Medicine and Environmental Assessment

Author : Jacob Kuruvilla
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Proteomics is evolving as a multi-faceted tool for addressing various biochemical and biomedical queries in the field of scientific research. This involves various stages, ranging from sample preparation to data analysis and biological interpretation. Sample preparation involves isolating proteins from the sample source, purifying and digesting them to initiate shotgun proteomics. Shotgun proteomics identifies proteins by bottom-up proteomic approaches where proteins are identified from the fragmentation spectra of their own peptides. Paper I: deals with the simplification of functional characterization for nanoparticles intended for use in biomedicine. Proteomics was constructive in differentiating and semi-quantifying the surface of protein corona. This could be beneficial in predicting the interactions between nanoparticles and a biological entity like the cell or a receptor protein and provide initial valuable information related to targeting, uptake and safety. Paper II: deals with understanding effects of TiO2 nanoparticles on endothelial cells. A combinatorial approach, involving transcriptomics and proteomics was used to identify aberrations in the permeability and integrity of endothelial cells and tissues. Our study also investigated the correlation of size and how they motivated a differential cellular response. In case of intravenous entry for nanoparticles in targeted drug delivery systems, endothelial cells are the first barrier encountered by these drug carriers. This evaluation involving endothelial cell response could be very instrumental during the designing of NP based drug delivery systems. Paper III: Pharmaceuticals and its metabolites could be very hazardous, especially if its disposal is not managed properly. Since water bodies are the ultimate sink, these chemicals could end up there, culminating in toxicity and other ‘mixture effects’ in combination with other factors. To evaluate the effects of the pharmaceutical, propranolol and climatic factors like low salinity conditions, a microcosm exposure was designed and shotgun proteomics helped understand its impact on mussel gills. In this study too, a combination of transcriptomics and proteomics unveiled molecular mechanisms altered in response to stressors, both individually and in combination. Paper IV: An interplay of various factors like EBF1 and PAX5 determines B-cell lineage and commitment. This might have been materialized by direct and transient proteinprotein interactions. A unique method called BioID helped screen relevant interactions in living cells by the application of a promiscuous biotin ligase enzyme capable of tagging proteins through biotinylation based on a proximity radius. Biotinylation of endogenous proteins enabled their selective isolation by exploiting the high affinity of biotin and streptavidin on streptavidin coated agarose beads, leading to their identification by mass spectrometry. The biotinylated proteins were potential candidate interactors of EBF1 and PAX5, which were later confirmed by sequencing techniques like ChIP-Seq, ATAC seq, and visualization techniques like proximity ligation assay (PLA).

Global Biodiversity

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Applied Genetics of Leguminosae Biotechnology

Author : Pawan K. Jaiwal
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Legumes include many very important crop plants that contribute very critical protein to the diets of both humans and animals around the world. Their unique ability to fix atmospheric nitrogen in association with Rhizobia enriches soil fertility, and establishes the importance of their niche in agriculture. Divided into two volumes, this work presents an up-to-date analysis of in vitro and recombinant DNA technologies for the improvement of grain, forage and tree legumes. Volume 10A examines the current status and future prospects of challenges of the following: in vitro morphogenesis; biotic and abiotic stress tolerance; genomics; nitrogen fixation and utilization; nutritional improvement, and biodiversity of wild and tribal legumes. Volume 10B presents the current state and future prospects of in vitro regeneration and genetic transformation expression and stability of transgenes modification of traits in almost all the important legumes, for example: soybean; peanut; pea; french bean; chick pea; pigeon pea; cowpea; mung bean; black gram; azuki bean; lentil; Lathyrus; lupinus; Lotus spp; Medicago spp; Trifolium spp; Winged bean; Guar; and tree legumes for their improvement. Written by international experts, these volumes will be of great value to researchers, as well as graduate students and all those requiring an advanced level overview of the subject area.

Proceedings of Symposium on Biotechnology in the Animal Health Sector Scientific Regulatory and Ethical Implications

Author : Jean D. Powers
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Eco Crime and Genetically Modified Food

Author : Reece Walters
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The GM debate has been ongoing for over a decade, yet it has been contained in the scientific world and presented in technical terms. Eco Crime and Genetically Modified Food brings the debates about GM food into the social and criminological arena. This book highlights the criminal and harmful actions of state and corporate officials. It concludes that corporate and political corruption, uncertain science, bitter public opposition, growing farmer concern and bankruptcy, irreversible damage to biodervisty, corporate monopolies and exploitation, disregard for social and cultural practices, devastation of small scale and local agricultural economies, imminent threats to organics, weak regulation, and widespread political and biotech mistrust – do not provide the bases for advancing and progressing GM foods into the next decade. Yet, with the backing of the WTO, the US and UK Governments march on – but at what cost to future generations?

Journal of World Trade

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Advances in Heat and Mass Transfer in Biotechnology

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Biotechnology

Author : Susan R. Barnum
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Biotechnology encompasses the processes and methods used to manipulate living organisms or the substances and products from these organisms for medical, agricultural, and industrial purposes. Barnum not only supplies the big picture of the biotechnology field, but provides in-depth details to illustrate the technology. Traditionally, biotechnology texts have been too narrow in coverage and focused only on the methods used in biotechnology. In contrast, Barnums text extensively covers the topics, with lots of examples and case studies, and discusses the implications in areas such as gene therapy, medicine, agriculture, marine biology, and forensics. Designed to grab the interest of students and make the topics relevant to them, Barnums text is an excellent integration of historical and modern biotechnology topics.

The Biological Weapons Convention

Author : Jez Littlewood
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The Federation of American Scientists (FAS) presents information about and the text of the Biological Weapons Convention, which was opened for signature on April 10, 1972. The treaty prohibits the development, production, stockpiling, or acquiring of biological agents or toxins among the United States, the Soviet Union, and Great Britain. The FAS provides information about the status, provisions, and history of the treaty.

Managing Agricultural Biotechnology

Author : Joel I. Cohen
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Although new technologies to improve agricultural production are constantly being devised, there is a great need to manage their development and implementation more effectively if they are to have a significant impact. Over the past few years ISNAR has been holding seminars and developing expertise in the management of agricultural biotechnology programs. This book provides information and case studies on the range of key managerial issues involved. It includes syntheses and review chapters, as well as case studies from a range of countries including Chile, India, Japan and the Philippines. The book consists of 24 chapters written by authorities from North, Central and South America, Europe, Asia and Australia and is written in an accessible style that will appeal to practicing managers as well as to academics.

Foundations of Space Biology and Medicine Space medicine and biotechnology

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Foundations of Space Biology and Medicine Volume 3 Space Medicine and Biotechnology

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Genetic Engineering Biotechnology News

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Intellectual Property Trade and Development

Author : Daniel Gervais
File Size : 26.82 MB
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This book provides a comprehensive analysis of the latest legal, economic, political and social research and advanced current thinking on the relationship between intellectual property and trade and development. It also sets out implementation strategies for the incorporation of intellectual property rules into a local legal framework.

International Environmental Liability and Barriers to Trade

Author : K. L. Holtby
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Trade and the environment has become a major issue in international relations, yet the surrounding debate remains polarised and hostile. This book answers the question: Can an international liability regime facilitate international trade while fostering environmental sustainability?On the one hand, the authors argue, international trade is perceived as a major threat to environmental sustainability, whilst on the other, trade and the economic development arising from it is seen as the prerequisite to stronger environmental protection. Nowhere is the debate more acrimonious than over trade in genetically modified organisms. The Biosafety Protocol has been negotiated to govern trade in genetically modified products and includes provisions for an international liability regime to facilitate trade - a subject that has been little studied. This valuable study explores the role, design and potential effectiveness of such a regulating body and addresses questions such as - what are the options for an international environmental liability regime? Why are some options unworkable? Is there a set of options that will achieve the dual goals of trade facilitation and environmental protection? Is international liability the best option for defusing the trade and environment debate?This illuminating book will be an essential read for scholars and students (senior undergraduate as well as postgraduate) of international trade policy, environmental economics, international politics and international law. Individuals working in international organizations, those employed by environmental NGOs and government policy makers will also find much to engage them within this book.

International Trade in GMOs and GM Products

Author : Simonetta Zarrilli
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This publication, one of a series of UNCTAD studies which discuss key international trade and development policy issues, focuses on the trade implications of the application of biotechnology techniques in agriculture and the use of genetically modified organisms (GMOs) and products derived from them. Topics considered include: domestic legislation on agro-biotechnology in selected developed and developing countries; the multilateral legal framework, including the Cartagena Protocol on Biosafety; actual and potential GM-related trade disputes.