Search results for: purifying-and-culturing-neural-cells

Purifying and Culturing Neural Cells

Author : Ben Barres
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Composed of countless neurons, glia, and vascular cells, the nervous system innervates all parts of the body to function as a vast communication network. This complexity makes it challenging to examine neural properties at the cellular and molecular levels. Cell culture systems for specific neural cell types are thus essential for studies of their development and function. This laboratory manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating cocultures of neurons and oligodendrocytes are also described. The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upward.

The Neuron in Tissue Culture

Author : L. W. Haynes
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This volume in the Handbook Series contains 73 contributions from experts in the range of fields to which neuronal tissue culture may be applied. Twenty-two keynote chapters contain methods, well-illustrated with data from the contributing authors? laboratones, which exemplify ?landmark? applications of the technique. Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum. The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book. The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons. It is essential reading for all those with research interests in neuroscience, including developmental biologists, neurologists, nerve cell physiologists and neuropharmaoologists.

Culturing Nerve Cells

Author : Gary Banker
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In many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols, but also providing an understanding of the principles behind the protocols. The contributors to this volume invite you to their labs and provide information

Research Awards Index

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Cumulated Index Medicus

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Research Grants Index

Author : National Institutes of Health (U.S.). Division of Research Grants
File Size : 33.31 MB
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Environmental Health Perspectives

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Psychopharmacology Bulletin

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The Growth and Differentiation of Nerve Cells in Culture

Author : Marilyn Anderson Ludueña
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Neuroscience Approached Thru Cell Culture

Author : Steven E. Pfeiffer
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Methods for Serum free Culture of Epithelial and Fibroblastic Cells

Author : David William Barnes
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Band 3.

Neuroscience Letters

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Neuron glia Interaction

Author : Victor Corvalan
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Neuronal and Glial Proteins

Author : Paul J. Marangos
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Neuronal and Glial Proteins ...

Methods for serum free culture of cells of the endocrine system

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The Journal of Neuroscience

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The Journal of Cell Biology

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No. 2, pt. 2 of November issue each year from v. 19-47; 1963-70 and v. 55- 1972- contain the Abstracts of papers presented at the annual meeting of the American Society for Cell Biology, 3d-10th; 1963-70 and 12th- 1972- .

Stem Cell Biology

Author : Jitka Ourednick
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Stem cells have generated considerable interest recently in the scientific, clinical, and public arenas. It is essential that we gain a broader understanding of the factors that regulate the biology of stem cells: their ability for self-renewal, differentiation and plasticity, as well as the differences between embryonic and adult stem cells. To gain a better understanding of how and whether stem cells can be manipulated to replace cells in diseased tissues will depend on gaining a better understanding of their normal developmental properties. This volume offers contributions from numerous interdisciplinary areas bridging biotechnology and biomedical sciences. The ability to isolate and maintain pluripotent stem cells in culture offers exciting possibilities for replacing damaged or diseased organs and tissues. Moreover, stem cells will provide opportunities for major advances in our understanding of fundamental developmental processes.

A Tunable Protein based Scaffold for the Study of Central Nervous System Regeneration

Author : Karin Straley
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A Report for the Year on the Research and Other Activities of the Division of Biology

Author : California Institute of Technology. Division of Biology
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