Search results for: t-cell-hybridomas

T Cell Hybridomas

Author : H.v. Boehmer
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For more than ten years cell fusion techniques have been applied in studies on various lymphocyte functions. Ig expression was first studied in hybrids obtained by fusing myeloma cells with fibroblasts (1) or lymphomas (2), both of which do not produce Ig, and with Ig producing myelomas (3) or human blood lymphocytes (4). Kohler and Milstein (5) fused a myeloma with spleen cells from immunized mice. Up to 10% of the hybrids obtained secreted antibodies specific for the immunizing antigen. This suggested that plasma cells preferenti ally fused with the myeloma cells, a finding which was of enormous practical value. It was found that both Band T lymphocytes could be fused with the T cell tumor BW5147, which is however not permissive for Ig synthesis (6). A very large number of T cell hybridomas were generated by fusing BW5147 with cell populations containing in vivo or in vitro activated cells (7). The hybrids showed no specific T cell functions and binding assays for T cell receptors were not available. In particular, no hybrids were obtained which expreS1ed specific cytolytic activity that could be tested in short-term Cr release assays (8). However, the frustrations expressed about these failures, published in January, 1978 (9), were relieved by Taniguchi and Miller's publication a few months later of T cell hybridomas producing antigen-specific suppressor factors (10). Unfortunately, their hybrids rapidly lost factor production.

Monoclonal Antibodies and T cell Hybridomas

Author : Günter J. Hämmerling
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T Cell Hybridomas

Author : Michael J. Taussig
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Monoclonal Antibodies and T cell Hybridomas

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Lymphocyte Hybridomas

Author : F. Melchers
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Hybridomas and Cellular Immortality

Author : Baldwin H. Tom
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The ability to "immortalize" immunologically-useful cells by hybridization with a unique cancer cell has revolutionized serological studies and has revealed new potential applications in all fields of biological sciences. This volume presents the studies from a highly successful national symposium on Hybridomas and Cellular Immortality held November 1981 in Houston, Texas. The individual chapters exhibit the diversity of topics discussed during the meeting. These include emphasis on the origin of antibody diversity, Band T lymphocyte differentiation, applications of monoclonal antibodies in studies of histocompatibility, tumor, and viral antigens, plus the use of somatic cell hybridizations for studying T cell products. Three papers focus on the emerging methodologies of in vitro primary immunizations for both humoral and cell-mediated immunities, relevant for coupling with hybridoma technology. There is a useful mix of general (methods) and specific (applications) chapters. A unique aspect of the book is the presentation of both recent research findings with concise descriptions of the state of the art methodologies. It is anticipated that this work will be of interest to a wide audience of practioners in biomedical research. Hopefully, the information contained will foster new and imagi native ideas in hybridoma applications. Baldwin H. Tom, Ph.D. James P. Allison, Ph.D. vii CONTENTS PART L INTRODUCTION TO HYBRIDOMAS 1 Somatic Cell Hybrids and Hybridomas Baldwin H. Tom 3 1. Somatic Cell Hybrids 8 Hybridomas. • • • • • 2.

Production and functional analysis of CD8 class I specific T cell hybridomas and studies of molecular mechanisms of T cell hybridoma activation

Author : Jing Jin Gu
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Isolation Characterization and Utilization of T Lymphocyte Clones

Author : C Fathman
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Isolation, Characterization, and Utilization of T Lymphocyte Clones is a summary of information regarding T lymphocyte clones, including their usefulness. Organized into nine parts, the book begins with discussions on the soluble factors that can influence the growth of cloned T cells and the utilization of T cell hybridomas for analysis of T cell functions, emphasizing the biochemical and functional properties of helper and suppressor factors. The book then looks into the analysis of T cell clones and hybridomas using techniques of somatic cell genetics. The clonal analysis by limiting dilution, the characteristics of murine T cell clones reactive with alloantigens and soluble antigens, and the human T cell clones are described as well. This volume is valuable to those interested in the field of cloning of immunocompetent T cells.

Human Hybridomas and Monoclonal Antibodies

Author : Edgar Engleman
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Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.

Current Topics in Microbiology and Immunology

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Lymphocytes

Author : Sarah L. Rowland-Jones
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Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

T Cell Protocols

Author : Kelly P. Kearse
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In T Cell Protocols: Development and Activation, Kelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced investigator. Coverage includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases. Practical and timely, T Cell Protocols: Development and Activation offers basic scientists and clinical investigators a valuable compendium of key protocols that will improve skills with existing methods, as well as afford rapid mastery of powerful new techniques.

Monoclonal Antibodies T Cell Products

Author : David H Katz
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First Published in 1982, this book offers a full, comprehensive guide into the applications of Monoclonal Antibodies. Carefully compiled and filled with a vast repertoire of notes, diagrams, and references this book serves as a useful reference for Students of Medicine, and other practitioners in their respective fields.

Lymphocytes A Practical Approach

Author : Sarah L. Rowland-Jones
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Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

Antigen presenting Cell Requirements of Autoreactive T cell Hybridomas in Experimental Autoimmune Uveoretinitis

Author : Shiv Anand Prasad
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Handbook of B and T Lymphocytes

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To compile this up-to-date handbook, Dr. Charles Snow of the University of Kentucky Medical Center has assembled works by a premier group of active investigators who meet the needs of todays researchers. The handbookprovides a broad overview of the historical and current research in B- and T-cell biology, and presents the latest developments in this fast-breaking field to all professionals concerned with the humoral immune response of the body.

Programmed Cell Death by T Cell Hybridomas in Response to Minor Lymphocyte Stimulatory Antigens

Author : Jennifer Payne
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Antigen Recognition by Poly 18 Specific T Cell Hybridomas

Author : Michel Boyer
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Monoclonal T Cells and Their Products

Author : Marc Feldmann
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Processing and Presentation of Antigens

Author : Benvenuto Pernis
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Processing and Presentation of Antigens brings together the views of immunologists, biochemists, cell biologists, and virologists who have a common interest in the early phases of the immune response. Antigen processing and presentation comprise a series of events that are much more complex and sophisticated than had been thought for a long time, and constitute an essential part of the biology of the immune response to T-dependent protein antigens. The book is organized into seven parts. Part I discusses the major advances and insights in antigen processing, some results on the interaction of antigen with class II MHC molecules, and the precise mechanism involved in antigen processing. Part II presents papers on endosomes, lysosomes, and recycling. Part III deals with studies on presentation in the context of class I MHC molecules. The chapters in Part IV examine interactions of antigens with class II MHC molecules. Part V contains papers on macrophages and dendritic cells as accessory cells. Part VI examines antigen presentation by B cells. Part VII covers studies on T cells.